拟南芥 NOTCHLESS 在根和胚胎发育中发挥重要作用,Plant Signaling & Behavior

抽象的

核糖体生物发生是真核细胞的基本过程。 NOTCHLESS (NLE) 参与酵母中的 60S 核糖体生物发生，但其在拟南芥 ( A. thaliana ) 中的作用仍然是独有的。在这里，我们发现拟南芥NLE （ AtNLE ）在系统发育上高度保守，它编码WD40重复蛋白。 AtNLE在活跃分裂的组织中表达。 AtNLE-GFP 位于细胞核中。 AtNLE 与 AtMDN1 的 MIDAS 结构域发生物理相互作用，AtMDN1 是拟南芥中参与 60S 核糖体亚基生物发生的蛋白质。表达不足的突变体nle-2显示根短且根分生组织中细胞数量减少。此外，无效突变体nle-1是胚胎致死的，有缺陷的胚胎在早期球形阶段被阻止。这项工作表明 AtNLE 与 AtMDN1 相互作用，并且 AtNLE 在根和胚胎发育中发挥作用。

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Arabidopsis NOTCHLESS plays an important role in root and embryo development

ABSTRACT

Ribosome biogenesis is a fundamental process in eukaryotic cells. NOTCHLESS (NLE) is involved in 60S ribosome biogenesis in yeast, but its role in Arabidopsis (A. thaliana) remains exclusive. Here, we found that Arabidopsis NLE (AtNLE) is highly conservative in phylogeny, which encoding a WD40-repeat protein. AtNLE is expressed in actively dividing tissues. AtNLE-GFP is localized in the nucleus. AtNLE physically interacts with the MIDAS domain of AtMDN1, a protein involved in the biogenesis of the 60S ribosomal subunit in Arabidopsis. The underexpressing mutant nle-2 shows short roots and reduced cell number in the root meristem. In addition, the null mutant nle-1 is embryo lethal, and defective embryos are arrested at the early globular stage. This work suggests that AtNLE interacts with AtMDN1, and AtNLE functions in root and embryo development.